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microarray hybridization protocols  (Illumina Inc)


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    Illumina Inc microarray hybridization protocols
    Microarray Hybridization Protocols, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+protocol/microarray+hybridization/pmc06547664__41421_2019_95_MOESM1_ESM-127-3-14
    Average 90 stars, based on 1 article reviews
    microarray hybridization protocols - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Anti-oxidative stress effect of red ginseng in the brain is mediated by peptidyl arginine deiminase type IV (PADI4) repression via estrogen receptor (ER) β up-regulation.
    Article Snippet: Aim of the study: Ginseng has been used as an anti-stress agent, and its active ingredient, ginsenoside, is similar in structure to estrogen.. However, the effect of ginseng on the stressed brain is not completely understood.. The aim of this study is to understand systematically how red ginseng (RG) affects gene expressions in the brain of immobilization (IMO) stressed mice to elucidate its underlying mechanism.

    Article Title: Comparative analysis of gene expression profiles of hip articular cartilage between non-traumatic necrosis and osteoarthritis.
    Article Snippet: a Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China b Department of Rheumatology, Xi'an Fifth Hospital, PR China c Department of Radiation Oncology, First Affiliated Hospital, Health Science Center, Xi'an Jiaotong University, PR China d Department of Orthopedics, Second Affiliated Hospital of Health Science Center of Xi'an Jiaotong University, Xi'an, Shaanxi, PR China

    Hybridization:

    Article Title: Anti-oxidative stress effect of red ginseng in the brain is mediated by peptidyl arginine deiminase type IV (PADI4) repression via estrogen receptor (ER) β up-regulation.
    Article Snippet: Aim of the study: Ginseng has been used as an anti-stress agent, and its active ingredient, ginsenoside, is similar in structure to estrogen.. However, the effect of ginseng on the stressed brain is not completely understood.. The aim of this study is to understand systematically how red ginseng (RG) affects gene expressions in the brain of immobilization (IMO) stressed mice to elucidate its underlying mechanism.

    Article Title: Comparative analysis of gene expression profiles of hip articular cartilage between non-traumatic necrosis and osteoarthritis.
    Article Snippet: a Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China b Department of Rheumatology, Xi'an Fifth Hospital, PR China c Department of Radiation Oncology, First Affiliated Hospital, Health Science Center, Xi'an Jiaotong University, PR China d Department of Orthopedics, Second Affiliated Hospital of Health Science Center of Xi'an Jiaotong University, Xi'an, Shaanxi, PR China

    Expressing:

    Article Title: Anti-oxidative stress effect of red ginseng in the brain is mediated by peptidyl arginine deiminase type IV (PADI4) repression via estrogen receptor (ER) β up-regulation.
    Article Snippet: Aim of the study: Ginseng has been used as an anti-stress agent, and its active ingredient, ginsenoside, is similar in structure to estrogen.. However, the effect of ginseng on the stressed brain is not completely understood.. The aim of this study is to understand systematically how red ginseng (RG) affects gene expressions in the brain of immobilization (IMO) stressed mice to elucidate its underlying mechanism.

    Article Title: Comparative analysis of gene expression profiles of hip articular cartilage between non-traumatic necrosis and osteoarthritis.
    Article Snippet: a Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China b Department of Rheumatology, Xi'an Fifth Hospital, PR China c Department of Radiation Oncology, First Affiliated Hospital, Health Science Center, Xi'an Jiaotong University, PR China d Department of Orthopedics, Second Affiliated Hospital of Health Science Center of Xi'an Jiaotong University, Xi'an, Shaanxi, PR China



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    Image Search Results


    (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Microarray, Expressing, Transfection, shRNA, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Recombinant, Microarray, shRNA, Software